Download e-book for iPad: Advances in Environmental Research, Volume 13 by Justin A. Daniels

By Justin A. Daniels

ISBN-10: 1612090494

ISBN-13: 9781612090498

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2006; Zhang and Fang, 2006). In addition qPCR is more sensitive than traditional PCR methods and can estimate the initial concentration of nucleic acids. As with standard PCR methods, multiplexing qPCR is limited by the difficulty in optimizing reactions so that all targets are amplified with similar efficiencies. Moreover, the number of assays that can be multiplexed is limited by the number of fluorescent dyes that can be distinguished in a single reaction (typically no more than 4, depending on the specifications of the instrument being used).

DNA spots are typically very small, on the micrometer length scale, and can be arrayed in high density with hundreds to thousands of spots present on a single array. Detection using microarrays is based on the hybridization of fluorescently labelled nucleic acids (RNA or DNA) isolated from the test matrix to these probes, and subsequent measurement of fluorescence at each spot. Microarrays allow the simultaneous detection of thousands of targets in a single assay. Thus, a single array could be used for the detection of a wide range of pathogens, virulent strains of a particular pathogen, antimicrobial resistant strains, and even molecular typing.

Coli in food and animal foodstuffs (Anon, 2001). In addition, different chromogenic media such as Chromocult Coliform agar (CC agar), and coli ID agar have been developed for the simultaneous detection of total coliforms and E. , 2000). , 2003). , 2006). The results obtained with the combination of the two media, Tergitol agar and TBX agar, for the enumeration of total coliforms and E. , 2006). , 2006). In addition, using 26 William J. Snelling, Catherine D. Carrillo, Colm J. Lowery et al. this small modification, the enumeration of E.

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Advances in Environmental Research, Volume 13 by Justin A. Daniels


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